L'hybridation moléculaire : le "Southern Blot"

Southern and northern blot fixing by microwave oven.

Southern and Northern blot techniques are powerful procedures for studying gene structure and function. These methods involve the separation of nucleic acids according to size on an agarose or polyacrylamide gel and subsequent transfer of the nucleic acid to suitable nitrocellulose or nylon-based membranes. D N A or R N A molecules bound to the membrane are then fixed and hybridized with a radi...

Eighteen Years of Molecular Genotyping the Hemophilia Inversion Hotspot: From Southern Blot to Inverse Shifting-PCR

The factor VIII gene (F8) intron 22 inversion (Inv22) is a paradigmatic duplicon-mediated rearrangement, found in about one half of patients with severe hemophilia A worldwide. The identification of this prevalent cause of hemophilia was delayed for nine years after the F8 characterization in 1984. The aim of this review is to present the wide diversity of practical approaches that have been de...

A southern blot protocol to detect chimeric nuclease-mediated gene repair.

Gene targeting by homologous recombination at chromosomal endogenous loci has traditionally been considered a low-efficiency process. However, the effectiveness of such so-called genome surgery or genome editing has recently been drastically improved through technical developments, chiefly the use of designer nucleases like zinc-finger nucleases (ZFNs), meganucleases, transcription activator-li...

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Characterization of Mycobacterium tuberculosis strains from Vietnamese patients by Southern blot hybridization.

A total of 41 Mycobacterium tuberculosis strains from patients of Vietnamese origin were analyzed by Southern blot hybridization with two different probes, IS6110 (Otal, I., et al., J. Clin. Microbiol. 29:1252-1254, 1991; Ross, B. C., et al., J. Clin. Microbiol. 30:942-946, 1992; Thierry, D., et al., J. Clin. Microbiol. 28:2668-2673, 1990; van Soolingen, D., et al., J. Clin. Microbiol. 29:2578-...